Traditional methods for detecting DNA amplicons, such as gel electrophoresis, are time-consuming, involve multiple pipetting steps that increase the risk of operator error, and require the handling of hazardous chemicals like Ethidium Bromide. These procedures are not only complex and inefficient but also pose safety risks and are unsuitable for use outside of well-equipped laboratories. There is a clear need for a simpler, faster, and safer alternative for DNA detection.

The EzGenostick is directed to a device for a simple and rapid visual detection of amplified DNA of a target gene using polystyrene microparticles coated with capture reagents and visualized through gold nanoparticle-based detector reagent within a bound glass membrane-based lateral-flow device.

The innovation of this project lies in the development of EzGenostick, a novel microsphere-based lateral flow device that enables the rapid detection of PCR-amplified DNA in as little as five minutes, without the need for specialised equipment or trained personnel. Unlike conventional methods, it eliminates the need for multiple preparation or washing steps, making the process much simpler and more user-friendly. Importantly, the device avoids the use of hazardous chemicals such as Ethidium Bromide, and does not rely on complex instruments like fluorescence-based PCR machines. It also features an internal amplification control, allowing for the validation of PCR assays and ensuring test accuracy. With a built-in operational control, EzGenostick represents a significant step forward in making DNA detection faster, safer, and more accessible, especially in point-of-care and low-resource settings.