Invasive alien fish species present a serious threat to Malaysia’s freshwater ecosystems, often outcompeting native species and causing long-term ecological damage. A major challenge in managing these species is the delay in detection, which frequently results in irreversible biodiversity loss. Current detection methods are time-consuming, require access to fully equipped laboratories, and are not suitable for rapid field use. This limits the ability of local authorities and researchers to respond quickly and effectively to emerging threats. There is a clear need for a fast, accurate, and portable DNA-based detection tool that enables early identification and on-site monitoring of invasive species, helping to prevent their spread and protect freshwater biodiversity.

The solution is a portable, DNA-based detection tool that uses Loop-mediated Isothermal Amplification (LAMP) technology for the rapid identification of invasive alien fish species in Malaysia’s freshwater ecosystems. This method enables on-site DNA amplification using Bst polymerase under constant temperature conditions, removing the need for laboratory-based thermal cycling equipment. It can also process crude biological samples, eliminating the requirement for full DNA extraction. Designed for field deployment, this tool allows for quick, accurate detection of invasive species, empowering authorities and researchers to take immediate action—supporting faster response times, reducing ecological damage, and improving biodiversity protection and ecosystem management.

This innovation is a portable DNA detection tool that uses LAMP (Loop-mediated Isothermal Amplification) to quickly identify invasive fish species in freshwater. Unlike traditional lab methods, it works in the field, without needing full DNA extraction or complex equipment. It uses Bst polymerase to amplify DNA at a constant temperature and can test crude samples directly, saving time and effort. Results are fast and reliable, helping local teams act quickly to stop the spread of harmful species. The system also has the potential to be developed into a compact biosensor, making it even easier to use for early detection and ecosystem protection.